WebDNA complex excitation maximum = 355 nm DNA complex emission maximum = 465 nm Product Description Hoechst 33342 (bisBenzimide H33342 trihydrochloride) is supplied as a lyophilized powder in 25 mg … WebApr 18, 2024 · Hoechst is widely used in co-staining applications that simultaneously visualize DNA and other cellular structures or specific proteins. Its excitation/emission spectra do not overlap with other commonly used small-molecule fluorophores and fluorescent proteins that emit in the green-red range.
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WebHoechst 33342 is a fluorescent compound with an excitation peak at 352 nm and an emission peak at 454 nm, giving it a fairly large Stokes' Shift of 102nm. It can be excited … WebThe Nikon dual excitation band DAPI-FITC filter set is designed for the simultaneous detection of the popular fluorophores DAPI and FITC (fluorescein isothiocyanate) when applied in combination, and … multi cherry pick
The Use of Hoechst Dyes for DNA Staining and Beyond - MDPI
WebEpi Red LED Excitation . Turns “ON” or “OFF” the 640 nm overhead Red lighting (for excitation of Cy5, Cy5.5 and Alexa 647) 8 . Epi UV Excitation . Turns “ON” or “OFF” the 365 nm overhead UV lighting (for excitation of QDot, wtGFP, Hoechst Blue and … WebExcitation emission spectrum of Hoechst dye. Both dyes are excited by ultraviolet light at about 350 nm and both emit blue-green fluorescence with a maximum emission spectrum of 461 nm. Unbound dyes have maximum fluorescence emission in the 510-540 nm range. Hoechst stains can be excited with xenon or mercury arc lamps or UV lasers. WebFor imaging Hoechst 33342 staining, a 405 nm laser was used for excitation (solid-state laser rated power = 100 mW) together with a blue 430–470 nm emission filter. Live/dead cell analysis was performed using IN Carta analysis software128 (GE Healthcare). multichen category work