WebAbbreviations: 3-MA: 3-methyladenine; ATG5: autophagy related 5; EndMT: endothelial-to-mesenchymal transition; HES: hematoxylin and eosin stain; HFD: high-fat diet; HMVECs: … WebScale bar:50 μm. (K) Quantification of mitochondrial membrane potential (JC-1 aggregate/monomer ration). (L–M) Western blot analysis of PTGS2, NCOA4, FTH1, LC3, p-ULK1, Atg5 and Atg7 in H9C2 cells cotreated with PLG and OA/PA for 24 h (n = 3, *p < 0.05 vs. indicated group). (For interpretation of the references to colour in this figure ...
Metformin attenuates sevoflurane-induced neurocognitive …
Webcovalently bound to Atg5 and targeted to autophagosome vesicles (4-6). This conjugation reaction is mediated by the ubiquitin-E1-like enzyme Atg7 and the E2-like enzyme Atg10 (7,8). Directions for Use: CST recommends transfection with 100 nM Atg5 siRNA I 48 to 72 hours prior to cell lysis. For transfection procedure, follow protocol provided by the WebSimilar to the in vitro data, endothelial-specific atg5 knockout mice (Atg5 Endo; Cdh5-Cre Atg5 flox/flox mice) displayed both EndMT-associated kidney and heart fibrosis when compared to littermate controls. The plasma level of IL6 was higher in Atg5 Endo compared to that of control mice, and fibrosis was accelerated in Atg5 Endo treated with a ... how can you preview the merge results
Atg5 Antibody - Cell Signaling Technology
WebNational Center for Biotechnology Information WebAug 7, 2024 · Briefly, 1 mg of Dynabeads was conjugated to anti-FADD (Santa Cruz, sc-6036), anti-ATG5 (CST, 8540) antibody, goat IgG control (Santa Cruz, sc-2028) or rabbit IgG (Jackson ImmunoResearch, Ely, UK, 011-000-003) overnight at 4 °C. Dynabeads were washed in ice-cold NP-40 lysis buffer and combined with cell lysates on a rotor at 4 °C … WebOverview. To promote an efficient marine transportation system by providing increased flexibility in the construction and operation of U.S. flag vessels, while maintaining … how can you prevent yeast infections